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THE PROJECT REMOVAS

Removing Arsenic from rice with microalgae

Rice is the main staple food source for billions of people worldwide, and As tends to accumulate in higher concentrations in this grain crop, more than in other grain crops, increasing the human health risk. Arsenic is one of the main toxic element found in the environment, as a result of natural geogenic and anthropic sources. Because its potential risk to the ecosystem and to human health the United States Environmental Protection Agency (USEPA) classifies inorganic As as a group A carcinogen and the Agency for Toxic Substances and Disease Registry (ATSDR) ranked As as number one in its 2019 Substance Priority List. High As concentrations in rice grain is a consequence of As bioavailability in soil and speciation under conditions in which rice is cultivated and own intrinsic ecophysiological behaviour of this species. This can be a result from soil reducing conditions by flooding as it from the use of irrigation waters with high As levels. Therefore, it is a major research goal to issue sustainable production strategies that create conditions for a decrease uptake of As in paddy fields and consequently decrease As accumulation in the rice grain. The growth of algae in rice fields has been considered a natural fertilization process, as decomposition of algae adds nitrogen and other nutrients to the paddy soil. Moreover, microalgae produce oxygen during its phototrophic growth, which could create conditions that allows shifts in the paddy water redox potential and pH, thus promoting oxidation of As(III) to As(V) and further methylation, decreasing the bioavailability of As for uptake. The purpose of this project is to explore the possibility of integrating microalgae cultures into the rice paddy fields farming system towards decreasing the bioavailability of As for uptake, without compromising rice yields and the agroecosystem. Moreover, the project will also explore the use of paddy fields microalgae as potential secondary sources of income for rice producers (e.g. production of bioenergy) and creating new differentiated products to the rice miller’s portfolio. The holistic value chain approach of this proposal, is in line with the new European Green Deal policy framework linked with the UN 17 Sustainable Development Goals for 2030, to promote a resource-efficient and competitive economy while guarantying the health and well-being of citizens from environment-related risks and impacts, covering the “farm to fork” food value chain.

The Project: Bem-vindo

OBJECTIVES AND THEMATIC LINES ADDRESSED

The objective is to evaluate the influence that microalgae produces in rice paddy redox conditions, to decrease the level of As in rice grains, and follow up the food value chain, finding an added value for the microalgae biomass and diversify the rice millers portfolio. Thematic line: Tropical Agriculture & Agri-Food Value Chains addressing the topic “Tropical agro-food systems”. The idea can be easily implemented on tropics as well as in Portugal. An agro-food system combining a microalgae and a food crop for a synergism on As uptake will be fundamental on tropical countries, struggling with high levels of As in irrigation water, such as Bangladesh, but it will also be useful in Iberian peninsula where some regions produce rice with As levels too close to the limits set up by EU legislation. The idea came out from a previous study dealing with microalgae and other studies about As behaviour in soil-rice system in different paddy soils from Portugal and Spain. LEAF is the right place to implement such a knowledge cross-cutting project, since it gathers scientific experts with large experience working with these issues, from all the LEAF research groups.

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The Project: Sobre

THE REMOVAS TEAM

PATRICIA E. D. VIDIGAL

PI
Abiotic Stress; Antioxidative system; Soil rehabilitation; Crop Production

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ISABEL MARIA NUNES DE SOUSA

Co-PI

Food Rheology; Sustainable Functional Foods product development

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ANTÓNIO GUERREIRO DE BRITO

resource recovery and biofilms - bioenergy, phosphorus, environmental engineering, water management and planning

agbrito@isa.ulisboa.pt
https://orcid.org/0000-0003-1356-3

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CRISTIANE OTTONI

Biotechnology, Fungi, Nanotechnology, Microbial Fuel Cell, Enzymes, Trametes versicolor, Wastewater treatment

cristiane.ottoni@unesp.br
https://orcid.org/0000-0003-4069-509X

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DAVID FANGUEIRO

Animal manure treatment, Agronomic valorization, Environmetal impact, Gaseous emissions

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EMÍLIA SILVA

pesticides, aquatic ecotoxicology, environmental contamination, environmental risk assessment

emiliasilva@isa.ulisboa.pt
https://orcid.org/0000-0002-7498-8926

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ERIKA S. SANTOS

Environmental rehabilitation, Biogeochemistry, Technosols, Phytostabilisation/Phytoremediation, Soil-plant system, Contaminated/degraded areas

erikasantos@isa.ulisboa.pt
https://orcid.org/0000-0002-3664-839X

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MARIA DA GLORIA ESQUIVEL

Photobioreactors; Nannochloropsis; Chlorella Sorokiniana; Ectothermy; Heat Tolerance; Acclimatization

gesquivel@isa.ulisboa.pt
https://orcid.org/0000-0003-1772-0015

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Mª JOSÉ CEREJEIRA

pesticides, aquatic ecotoxicology, environmental contamination, environmental risk assessment

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Mª LUÍSA LOURO MARTINS

edible plants biochemistry, bioactive compound, chemical contaminants, abiotic stress, antiox

luisalouro@isa.ulisboa.pt
https://orcid.org/0000-0002-9486-6053

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Mª MANUELA ABREU

Phytoremediation, mines, metals, trace elements

manuelaabreu@isa.ulisboa.pt
https://orcid.org/0000-0002-6650-1161

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MIGUEL MOURATO

Heavy metals, oxidative stress, antioxidative enzymes

mmourato@isa.ulisboa.pt
https://orcid.org/0000-0003-4843-1064

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SERRA PAIXÃO

PhD Student

Serra is an aquaculture engineer. She holds a master`s degree in water quality & chemistry as well as an MBA in international marketing & foreign trade. After working for several years as an engineer with a focus on RAS design and environmentally sustainable aquaculture practices, she started to pursue a career in marketing within Ireland, Netherlands and Portugal. She is currently working as a Project Manager at a non-for-profit business association in off-grid solar sector and is a Ph.D. student at the Instituto Superior de Agronomia, University of Lisbon - LEAF Research Center.

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The Project: Serviços

PROJECT OUTPUTS

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GREENFEST BRAGA 2021

Our project was presented on the major event dedicated to sustainability - Greenfest 2021.

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OTTONI ET AL.
VEGU 2021

Cristiane Ottoni presented at vEGU2021, RemovAs vision to use rice-microalgae As removal to generate electricity using a microbial  fuel cell. This is a new conceptual approach for the agro-industrial sector

The Project: Projetos
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FUNDING AND OUR RESEARCH CENTER

https://www.isa.ulisboa.pt/en/leaf/presentation

Funded by national funds through FCT in the scope of the project LEAF Research Unit Ref. UIDB/04129/2020

The Project: Título
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The Project: Imagem
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